Mouse Anti-Modified citrulline Recombinant Antibody (clone 4CS)
CAT#: ZG-453C
This product is a mouse antibody that recognizes modified citrulline. It can be used for ELISA and WB.
Tested Data
ELISA
Figure 1 Mouse Anti-Modified citrulline Antibody (ZG-453C) in ELISA
Antibody diluted to 0.4 μg/ml was tested on various modified citrulline-containing peptides and their non-modified forms.
WB
Figure 2 Mouse Anti-Modified citrulline Antibody (ZG-453C) in WB
A) After blotting, the proteins on the membrane (fibrinogen, soybean trypsin inhibitor (STI), histones and their de-iminated forms) were chemically modified with 2,3-butanedione monoxime and antipyrine. Subsequently, the membrane was probed with AMC antibody. The right panel shows the corresponding SDS-PAGE gel stained with coomassie. B) AMC antibody was tested on 10 μg HEp-2 cell lysate and 10 μg of its de-iminated form after chemical modification of the proteins on the membrane with 2,3-butanedione monoxime and antipyrine (left panel). As control (de-iminated) STI was loaded. The right panel shows the corresponding SDS-PAGE gel stained with coomassie.
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Specifications
- Immunogen
- Citrulline-containing peptide modified with 2,3-butanedione monoxime and antipyrine
- Host Species
- Mouse
- Type
- Mouse IgG2a, κ
- Specificity
- Recognizes citrulline-containing proteins modifiedwith 2,3-butanedione monoxime and antipyrine regardless of neighbouring amino acid sequences.
- Clone
- 4CS
- Applications
- ELISA, WB
Product Property
- Purification
- Protein A affinity purified
- Purity
- >95% as deterimined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS
- Storage
- Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.
Target
- Alternative Names
- Citrulline, modified
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To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# ZG-453C, RRID: AB_3112069)
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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COA
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See other products for "Modified citrulline"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-454C | Human Anti-Modified citrulline Recombinant Antibody (clone C4) | ELISA, WB | Human IgG1, λ |
| ZG-455C | Human Anti-Modified citrulline Recombinant Antibody (clone C4S) | ELISA, WB | Human IgG1, κ |
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